沈慧 秦海宏 龙建纲 王福俤 郭俊生
摘要：目的 研究锌对Caco2细胞ZIP4 mRNA表达的影响及其规律。方法 通过锌特异螯合剂TPEN建立低锌Caco2细胞模型，RT-PCR法获得ZIP4 cDNA片断，10μM TPEN培养基诱导后，分别检测0、2、4、6、8和10h时点ZIP4 mRNA的表达，及0、2.5、5、7.5、10μmol/L TPEN培养基诱导6h，检测各浓度组ZIP4 mRNA的表达。结果 RT-PCR获得单一条带的片断，大小与设计一致，获得正确的ZIP4 cDNA片断，随着低锌时间的增加，ZIP4 mRNA表达也升高，6h达到峰值；随着TPEN浓度的升高，ZIP4 mRNA表达也随之升高。结论 ZIP4 mRNA表达受锌的调控，提示可能参与小肠对锌的吸收。
关键词： 锌 锌转运体 锌铁调控蛋白
中图分类号：R151.3 R735 文献标识码：A
Effect of zinc on mRNA expression of ZIP4 in Caco2 cells
Shen Hui, Qin Hai-hong, Long Jian-gang, Wang Fu-di, et al.
Department of Military Hygiene, Second Military Medical University, Shanghai 200433, China
Abstract: Objective To study the effect of zinc on mRNA expression of ZIP4 in human intestinal Caco2 cells and its regularity. Methods Low zinc cell model was established by TPEN, a kind of chelating agent which chelates specially to zinc. ZIP4 cDNA fragment was obtained by RT-PCR. Expression of ZIP4 on 10μM TPEN exposure after 0, 2, 4, 6, 8 and 10 hours in Caco2 cells and its expression on various concentration of TPEN exposure (0,2.5,5,7.5 and 10μmol/L) was measured by RT-PCR. Results A proper single fragment is obtained with the sequence conformable to the design. A proper single ZIP4 cDNA fragment was obtained. The mRNA expression of ZIP4 increased in accordance with the duration of low zinc. The peak mRNA level appeared at about 6h. And the ZIP4 mRNA increased in accordance with the concentration of TPEN in Caco2 cells. Conclusion Zinc can regulate the mRNA expression of ZIP4 in Caco2 cells. And ZIP4 may play a role in the absorption of zinc in human intestine.
Key words: zinc, zinc transporter, ZRT,IRT-like protein
杨莉 杨维东 刘洁生1 江涛
摘要：目的 对广州市售双壳经济贝类麻痹性贝毒（PSP）和腹泻性贝毒（DSP）污染状况进行为期一年的抽样调查，了解其食用安全性。方法 采用AOAC推荐的小鼠生物检测法进行PSP和DSP的毒力测定，采用HPLC进行PSP成分分析，根据FAO、日本和欧盟水产食品卫生要求及我国渔政渔港监督管理局制订的贝类安全食用标准对贝类水产品的食用安全性进行评价。结果 在所调查的7种贝类中，有2种染有PSP，毒素含量在安全食用范围内，毒力大小随季节而变化，春冬两季含量相对较高；7种贝类中有6种共计36个样品染有DSP，有10个样品毒素含量超出安全食用标准，春冬两季染毒率较高。结论 广州市售贝类PSP含量和检出率整体水平较低；DSP检出率稍高，毒素含量也较高，应引起有关部门的关注，加强DSP监测工作。
关键词：麻痹性贝毒 腹泻性贝毒 贝类毒素 监测
Analysis of paralytic shellfish poisoning and diarrhetic shellfish poisoning of bivalves in seafood market of Guangzhou
Yang Li, Yang Wei-dong, Liu Jie-sheng, Jiang Tao
College of Life Science and Technology, Jinan University, Guangzhou 510632,China
Abstract：Objective Paralytic shellfish poisoning (PSP) and diarrhetic shellfish poisoning (DSP) in 7 species of economical shellfishes were analyzed for 1 year, which collected from Huangsha seafood market of Guangzhou from Apr, 2004 to Mar, 2005. Methods The levels of PSP and DSP in bivalves were determined with mouse bioassay of AOAC. The risk assessment of PSP and DSP in bivalves was conducted according to FAO and Chinese Administration Organization of Fish Culture and Seaport. Results PSP was detected in 2 species of the shellfishes assayed and DSP was found out in 6 species. The content of PSP was lower than 4MU/g tissue, whereas the level of PSP in glands was higher than in muscles. DSP toxin was detected in 36 samples of 6 species, what is more, DSP level in 10 samples exceeded the safety threshold. The levels in PSP and DSP of bivalves were all higher in spring and winter with some characteristic of season. Conclusin The results suggested that PSP in economical shellfish in Guangzhou market was lower, and shellfish was safe to eat in term of the PSP level if glands were discarded, but DSP contamination in bivalves was severe. It is essential to detect and assess the risk of DSP and PSP in bivalves from seafood market in the future.
Key words：paralytic shellfish poisoning, diarrhetic shellfish poisoning, toxins, detection
基金项目：国家重点基础研究发展规划项目“973” (No.2001CB409710，.2001CB409709 )；广东省科技计划项目(No.2003C20412)；广州市科技计划项目(No.2002J1-C0011)
娄晓民 段广才1 陈姜1 周艳1 胡巧云1 衡正昌
摘要：目的 分析儿童肥胖形成中肥胖发生调节因子及与青春期发育相关激素的变化，探讨这些激素在青春期儿童肥胖形成中的变化特点及其相互关系。方法 以78例肥胖的13岁儿童和84例同岁正常对照儿童为研究对象，以放射免疫法测定血中食欲素（orexins）、瘦素（leptin）、胰岛素（insulin） 及睾酮（T）、雌二醇（E2）、黄体生成素（LH）、卵泡刺激素（FSH）等浓度，计算骨龄（BA）。结果 肥胖儿童血中leptin、Insulin浓度显著高于对照组浓度（P<0.01）；肥胖儿童血中orexinA浓度显著低于对照组浓度（P<0.01）；肥胖男生T显著低于对照组浓度，肥胖女生E2、LH显著高于对照组浓度（P<0.01）；男女肥胖儿童骨龄明显大于对照组，且随着骨龄的增大血中Leptin, LH含量明显增加；男女肥胖与正常儿童LH与FSH之间、leptin与insulin, C-肽, E2之间均存在正相关关系P<0.01；对照组男生LH, FSH与E2存在正相关关系（r=0.373，0.314，P<0.05）；leptin与T存在负相关关系（r=-0.423，P<0.01），与FSH存在正相关关系（r=0.308，P<0.05）；对照组女生leptin, LH与E2均存在正相关性（r=0.585, 0.647，P<0.01）；男女肥胖组LH、FSH与T、E2之间均未发现相关关系，肥胖男生leptin与T呈负相关关系（r=-0.574，P<0.01）。结论 肥胖儿童相对与正常儿童血中leptin、orexinA、insulin和T、E2、LH浓度发生了明显变化，肥胖儿童高浓度leptin水平可使体内T, E2, LH浓度水平发生变化，影响肥胖儿童的青春期发育。
关键词：食欲素 瘦素 胰岛素 睾酮 雌二醇 黄体生成素 肥胖
中图分类号：R179 Q575.11 文献标识码：A
Study on the level changes of obesity-associated factors and puberty-associated hormones and their relationship between obese and normal 13 years old children
Lou Xiao-min, Duan Guang-cai, Chen Jiang, Zhou Yan, et al.
West China School of Public Health, Sichuan University, Chengdu 610041, China
Abstract : Objective To investigate the level changes of obesity-associated factors and puberty-associated hormones and their relationship between obese and normal Children. Methods The concentrations of orexins, leptin, insulin, testosterone(T), estradiol(E2), luteotrophic hormone(LH), as well as Follicle stimulating hormone(FSH) in blood were measured respectively in 78 cases of 13 years old obesity children and 84 normal children as a control group by the means of RIA methods. The bone ages were evaluated with “bone age standard of hundred percent scale”. Results The leptin and insulin concentrations were significantly higher in obesity children as compared with the control group （P<0.01）. The concentrations of plasma orexinA were significantly lower in obesity children as compared with the control group（P<0.01）. The concentrations of T were significantly lower in obesity boys as compared with the control group （P<0.01）. The concentrations of E2 and LH were significantly higher in obesity girls as compared with the control group （P<0.01）. The bone age of the obese children was significantly older than that of the control group. Along with the bone age of children became older, the concentration of leptin and LH in blood were significantly increased; There was a positive correlation between LH and FSH in two groups of either boys and girls and so did between leptin and Insulin, C- peptide, E2. P<0.01；In the group of normal boys, the positive correlations between LH, FSH and E2 were shown（r=0.373，0.314，P<0.05）, and a negative correlations between leptin and T were shown as（r=-0.423，P<0.01）,a positive correlation between leptin and FSH was also shown as （r=0.308，P<0.05）. In the control group of girls, there were positive correlations between leptin, LH and E2（r=0.585，0.647，P<0.01）. Any correlations between LH, FSH and T, E2 were not shown in the two obese groups of boys and girls. Conclusion The leptin, orexinA, Insulin, T, E2, and LH concentrations had been changed significantly in blood among those obesity children, and so as their interactions. The high level of leptin in obesity children may change the concentrations of T, E2 and LH and affect the development of puberty.
Key words :orexins，leptin，insulin，luteotrophic hormone，testosterone，estradiol，obesity